An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.
©2000 Optical Society of America
Hoeling, BM, Fernandez, AD, Haskell, RC, Huang, E, Myers, WR, Petersen, DC, Ungersma, SE, Wang, R, Williams, ME, Fraser, SE. An optical coherence microscope for 3-dimensional imaging in developmental biology. Opt Expr. 2000;6(7): 136-146.