Graduation Year

2014

Document Type

Open Access Senior Thesis

Degree Name

Bachelor of Science

Department

Biology

Second Department

Biology-Chemistry

Reader 1

Alan Jones

Reader 2

John H Morrison

Terms of Use & License Information

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 License.

Rights Information

© 2014 Sebastian Aguiar

Abstract

The excitatory, ionotropic glutamatergic AMPA receptor is the most common membrane-bound receptor in the central nervous system. AMPARs and the NMDA receptors are central to synaptic plasticity, memory, and mechanisms of neurodegeneration. The AMPAR is an obligate heterotetramer, composed of subunits GluA1-4. Subunit permutation determines ion conductance, trafficking and other functional characteristics. Few available antibodies are subunit-specific, disabling researchers from accurately visualizing differential AMPAR subunit distribution in the nervous system. This study sought to visualize a novel monoclonal GluA1/2/3 antibody with functional avidity for three of four receptor subunits and to characterize the ultrastructural localization of these receptors using confocal and electron microscopy.

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