Graduation Year

2014

Document Type

Campus Only Senior Thesis

Department

W.M. Keck Science Department

Second Department

Biology

Reader 1

Bryan Thines

Rights Information

© 2014 Paloma Medina

Abstract

Flowering in Arabidopsis thalina is controlled by multiple pathways and is repressed by cold. To understand how A. thalina molecularly responds to cold stress temperatures, we subjected seedlings to different temperatures of cold stress for four days. Specifically, we conducted quantitative PCR of CONSTANS (CO) and the F-Box protein FKF1 to observe specifically how cold temperatures might affect the flowering time of a plant. We found a 16°C cold stress temperature increased both CO and FKF1 transcription when compared to a 24°C control. The increased expression of both CO and FKF1 may serve as a priming pathway that enables plants to be ready to flower at the precise moment when spring arrives.

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