Graduation Year

2020

Date of Submission

12-2019

Document Type

Campus Only Senior Thesis

Degree Name

Bachelor of Arts

Department

Biochemistry

Reader 1

Ethan Van Arnam

Reader 2

Babak Sanii

Terms of Use & License Information

Terms of Use for work posted in Scholarship@Claremont.

Abstract

Defensive symbioses, in which microbes provide molecular defenses for an animal host, hold great potential as untapped sources of therapeutically useful antibiotics. Fungus-growing ants use antifungal defenses from bacterial symbionts to suppress pathogenic fungi in their nests. Preliminary chemical investigations of symbiotic bacteria from this large family of ants have uncovered novel antifungal molecules with therapeutic potential, such as dentigerumycin and selvamicin.

In this study, the bacterial symbionts of North American Trachymyrmex fungus-growing ants are investigated for antifungal molecules. Plate-based bioassays using ecologically-relevant fungal pathogens confirmed that these bacteria have antifungal activity. In order to purify and identify the antifungal molecules produced by a single strain we are using reversed-phase liquid chromatography for activity-guided fractionation. Preliminary mass spectrometry data suggests this is a novel compound. Identification of the antifungal molecules will allow us to assess their structural novelty, therapeutic potential, and to contextualize antifungal defense in nature.

This thesis is restricted to the Claremont Colleges current faculty, students, and staff.

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