Graduation Year

2019

Date of Submission

12-2019

Document Type

Open Access Senior Thesis

Degree Name

Bachelor of Arts

Department

Biophysics

Second Department

Physics

Reader 1

Jeff Urbach

Reader 2

Sarah Marzen

Terms of Use & License Information

Terms of Use for work posted in Scholarship@Claremont.

Rights Information

© 2019 Kelly Watanabe

Abstract

Giardia lamblia is a single-celled protozoan parasite that when ingested, causes diarrheal disease and infects 33% of people in developing countries. Previous studies observe Giardia in water-like fluids, but Giardia's infectious environment consists of viscoelastic mucus in the small intestine. Therefore, Giardia was cultured in viscoelastic fluids, and its population growth was observed in vitro. To create shear-thinning viscoelastic fluids, 0.2% and 0.4% long-chain polyacrylamide (LCPAM) was added to cell culture media. Giardia was cultured in control media, 0.2% LCPAM, and 0.4% LCPAM, and population growth was quantitatively determined over time. Increasing LCPAM concentration resulted in a solution with higher viscosity and elasticity. Experimental results suggest that Giardia growth is delayed in more viscoelastic fluids, but the population adjusts to the viscoelastic environments over time.

Available for download on Tuesday, June 08, 2021

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