Researcher ORCID Identifier
Date of Submission
Campus Only Senior Thesis
Bachelor of Arts
Zhaohua Irene Tang
2021 Gabriela Mejia
The Schizosaccharomyces pombe Dis1-suppressing protein kinase (Dsk1) and Kic1/Lkh1 protein play vital roles in regulating cell growth. Although Dsk1 is involved in chromosome segregation and cytokinesis, its molecular function is poorly understood. Results published from our lab suggest that serine/threonine protein phosphatase (Clp1) is a potential substrate of Dsk1. This study aims to investigate the changes in cellular localization and phosphorylation status of the Clp1 protein in the wild-type, the dsk1 and kic1 deletion strains. By means of fluorescence microscopy, we found that the localization of the GFP-tagged proteins is observed in the nucleus of all strains. Based on preliminary results of the phosphorylation status of Clp1, it is unclear whether either kinase affects Clp1. As precise chromosome segregation at mitosis is key for genomic stability and thus cancer prevention, understanding the molecular basis of Dsk1 and Clp1 functions in the cell cycle will provide helpful information about cancer development and treatment.
Mejia, Gabriela, "The Effects of Dsk1 and Kic1 Kinase on Phosphatase Clp1 in Mitotic Regulation" (2022). CMC Senior Theses. 2804.
This thesis is restricted to the Claremont Colleges current faculty, students, and staff.