Graduation Year


Document Type

Open Access Senior Thesis

Degree Name

Bachelor of Arts



Reader 1

Natalie Rubio

Reader 2

Emily Wiley

Terms of Use & License Information

Terms of Use for work posted in Scholarship@Claremont.

Rights Information

© 2022 Julian M Cohen


Cellular agriculture is an emerging technology aiming to replace existing methods for animal agriculture with tissue engineering and cell culture-based technologies. Cultured meat falls within this purview, using a biomimetic approach to recreate animal muscle tissue through tissue engineering. In the attempt to diminish the necessity of animal-derived materials within this process, plant-based scaffolds can be used as a substrate upon which stem cells are cultured. Due to the unfavorable environment of cellulose for mammalian cell-surface proteins, the approach was taken of coating cellulose nanofiber films with a fusion protein composed of a cellulose binding domain (CBD) protein and the cell-adhesion peptide motif RGD, upon which bovine satellite cells were then cultured. Using this protein as an intermediate upon which each component can bind, our results indicate statistically-significant enhancement of cell attachment within this system when using an FBS-containing media formulation.