Researcher ORCID Identifier


Graduation Year


Document Type

Open Access Senior Thesis

Degree Name

Bachelor of Arts



Reader 1

Bethany Caulkins

Reader 2

Mary Hatcher-Skeers

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Terms of Use for work posted in Scholarship@Claremont.

Rights Information

@2021 Julia R Didziulis


Ubiquitination and other post-translational modifications play a major role in the proliferation of many neurodegenerative diseases, developmental disorders, and cancers, and as such are subjects of recently increased biochemical interest. Expanding upon such research, this study confirmed a robust method of ubiquitin expression and purification, then used 15N-1H HSQC to analyze ubiquitin samples containing varying concentrations of a deep cavitand with affinity for lysine and arginine side chains. In the pure ubiquitin spectrum, arginine side chain chemical shifts were detected in the nitrogen 80-85 ppm range and lysine side chain signals were undetected. However, upon introduction of the cavitand, collection of chemical shift perturbation data was prohibited by the cavitand precipitating out of solution. To overcome this obstacle, future experiments may benefit by refining the purification protocol via size-exclusion chromatography or adjusting sample properties such as pH, salt content, and more. Once complete, these experiments may indicate the cavitand’s viability as a biosensor for ubiquitination and other modifications, possibly accelerating diagnostics and disease research as a result.