Graduation Year


Document Type

Open Access Senior Thesis

Degree Name

Bachelor of Arts


Organismal Biology

Reader 1

Pete Chandrangsu

Reader 2

Katie Purvis-Roberts


The canals of Bangkok, Thailand hold significant economic and social value, yet the increasing urbanization of the city has resulted in increased water pollution. Agricultural runoff and urban waste contribute to the degradation of the water quality, which has impaired its safe usage by the people of the city. This study focused on analyzing the microbiome of the water in the canals in correlation with the surrounding environment, both in and out of the water. Ten sites along the Bangkok canals were analyzed. DNA was isolated for the sequencing of the 16s rRNA gene to determine the microbial diversity of the area and its relation to the water quality. Sites 8, 9, and 10, which were located further inland, were found to consist of a different microbiome compared to sites 1 through 7. The conductivity, total dissolved solids, and salinity were also different between the two groups. Beta diversity analysis suggests that location along the canal likely plays a strong role in determining the microbiome of the water. Understanding the interaction of the water chemistry with the microbiology of the canals allows for local communities to better enact measures to improve the water quality.