Graduation Year
2019
Date of Submission
4-2019
Document Type
Open Access Senior Thesis
Degree Name
Bachelor of Arts
Department
Biology
Reader 1
Dr. Kiana Aran
Reader 2
Dr. John Milton
Terms of Use & License Information
Rights Information
© 2019 Kandace K.T. Fung
Abstract
Sickle Cell Disease (SCD) is a hereditary monogenic disorder that affects millions of people worldwide and is associated with symptoms such as stroke, lethargy, chronic anemia, and increased mortality. SCD can be quickly detected and diagnosed using a simple blood test as an infant, but as of now, there is currently limited treatment to cure an individual of sickle cell disease. Recently, there have been several promising developments in CRISPR-Cas-associated gene-editing therapeutics; however, there have been limitations in gene-editing efficiency monitoring, which if improved, could be beneficial to advancing CRISPR-based therapy, especially in SCD. The CRISPR-Chip, a three-terminal graphene-based field effect transistor (gFET), was used to detect genomic samples of individuals with SCD, with and without amplification. With the dRNP-HTY3’ complex, CRISPR-Chip was able to specifically detect its target sequence with and without pre-amplification. With the dRNP-MUT3’ complex, CRISPR-Chip was only able to specifically detect one of its two target sequences. Facile detection, analysis, and editing of sickle cell disease using CRISPR-based editing and monitoring would be beneficial for simple diagnostic and gene-editing therapeutic treatment of other single nucleotide polymorphisms as well, such as beta-thalassemia and cystic fibrosis.
Recommended Citation
Fung, Kandace, "Detection of Sickle Cell Disease-associated Single Nucleotide Polymorphism Using a Graphene Field Effect Transistor" (2019). CMC Senior Theses. 2262.
https://scholarship.claremont.edu/cmc_theses/2262
Included in
Analytical, Diagnostic and Therapeutic Techniques and Equipment Commons, Biology Commons, Biotechnology Commons
