Researcher ORCID Identifier

0000-0002-7636-1326

Graduation Year

2022

Date of Submission

12-2021

Document Type

Campus Only Senior Thesis

Degree Name

Bachelor of Arts

Department

Biology

Reader 1

Zhaohua Irene Tang

Reader 2

Erin Jones

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2021 Gabriela Mejia

Abstract

The Schizosaccharomyces pombe Dis1-suppressing protein kinase (Dsk1) and Kic1/Lkh1 protein play vital roles in regulating cell growth. Although Dsk1 is involved in chromosome segregation and cytokinesis, its molecular function is poorly understood. Results published from our lab suggest that serine/threonine protein phosphatase (Clp1) is a potential substrate of Dsk1. This study aims to investigate the changes in cellular localization and phosphorylation status of the Clp1 protein in the wild-type, the dsk1 and kic1 deletion strains. By means of fluorescence microscopy, we found that the localization of the GFP-tagged proteins is observed in the nucleus of all strains. Based on preliminary results of the phosphorylation status of Clp1, it is unclear whether either kinase affects Clp1. As precise chromosome segregation at mitosis is key for genomic stability and thus cancer prevention, understanding the molecular basis of Dsk1 and Clp1 functions in the cell cycle will provide helpful information about cancer development and treatment.

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