Graduation Year

2022

Date of Submission

4-2022

Document Type

Campus Only Senior Thesis

Degree Name

Bachelor of Arts

Department

Biophysics

Reader 1

Janet Sheung

Reader 2

Mary Hatcher Skeers

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Terms of Use for work posted in Scholarship@Claremont.

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© 2022 Marilyn Liriano

Abstract

Microscopy has long been heralded in the Biological Sciences for its revelations concerning the structures and the processes of cells. Progressively, new discoveries in the field of microscopy emerged and advanced the understanding of cells. The discovery of fluorescent dyes and proteins broadened the use of imaging. Methods that can observe fluorescence without harming the sample through radiation are invaluable for live studies. Various techniques have evolved including, Super Resolution microscopy and Light Sheet Fluorescence microscopy to optically section the sample without causing photo damage to the samples. An overview of advances in microscopy show the benefits of optical sectioning and its potential for imaging outside of life sciences in fields such as material science. Methods to manipulate the system for improved resolution are presented. The future of microscopy relies on educating students on the most basic concepts of optics and allowing them to build their own experiments to garner a thorough understanding of microscopes.

This thesis is restricted to the Claremont Colleges current faculty, students, and staff.

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