An Optical Coherence Microscope for 3-Dimensional Imaging in Developmental Biology

Authors

Barbara M. Hoeling, Harvey Mudd College
Andrew D. Fernandez, Harvey Mudd College
Richard C. Haskell, Harvey Mudd CollegeFollow
Eric Huang, Harvey Mudd College
Whittier R. Myers, Harvey Mudd College
Daniel C. Petersen, Harvey Mudd College
Sharon E. Ungersma, Harvey Mudd College
Ruye Wang, Harvey Mudd College
Mary E. Williams, Harvey Mudd College
Scott E. Fraser, California Institute of Technology

Document Type

Article

Department

Physics (HMC)

Publication Date

3-27-2000

Abstract

An optical coherence microscope (OCM) has been designed and constructed to acquire 3-dimensional images of highly scattering biological tissue. Volume-rendering software is used to enhance 3-D visualization of the data sets. Lateral resolution of the OCM is 5 mm (FWHM), and the depth resolution is 10 mm (FWHM) in tissue. The design trade-offs for a 3-D OCM are discussed, and the fundamental photon noise limitation is measured and compared with theory. A rotating 3-D image of a frog embryo is presented to illustrate the capabilities of the instrument.

Comments

First published in Optics Express, vol. 6, no. 7 (March 2000), by the Optical Society of America.

Rights Information

©2000 Optical Society of America

Terms of Use & License Information

Terms of Use for work posted in Scholarship@Claremont.

Recommended Citation

Hoeling, BM, Fernandez, AD, Haskell, RC, Huang, E, Myers, WR, Petersen, DC, Ungersma, SE, Wang, R, Williams, ME, Fraser, SE. An optical coherence microscope for 3-dimensional imaging in developmental biology. Opt Expr. 2000;6(7): 136-146.