Immunogold Labeling to Enhance Contrast in Optical Coherence Microscopy of Tissue Engineered Corneal Constructs

Authors

Chris B. Raub
Elizabeth J. Orwin, Harvey Mudd CollegeFollow
Richard C. Haskell, Harvey Mudd CollegeFollow

Document Type

Conference Proceeding

Department

Physics (HMC)

Publication Date

9-2004

Abstract

Our lab has used an optical coherence microscope (OCM) to assess both the structure of tissue-engineered corneal constructs and their transparency. Currently, we are not able to resolve cells versus collagen matrix material in the images produced. We would like to distinguish cells in order to determine if they are viable while growing in culture and also if they are significantly contributing to the light scattering in the tissue. In order to do this, we are currently investigating the use of immunogold labeling. Gold nanoparticles are high scatterers and can create contrast in images. We have conjugated gold nanoparticles to antibodies specific to the α₅ β₁ integrins expressed in some corneal cells. This choice of target should allow assessment of the phenotypic behavior of the cells in the tissue, as different integrins are expressed in different phenotypes. This study applies the immunogold technique to study cultured corneal cells using the OCM with the ultimate goal of monitoring cellular behavior in engineered tissue and corroborating results from standard histological methods.

Comments

Previously linked to as: http://ccdl.libraries.claremont.edu/u?/irw,353.

From the 26th Annual International Conference of the IEEE-Engineering-in-Medicine-and-Biology-Society [September 01-05, 2004, San Francisco, CA] IEEE Engn Med & Biol Soc, Whitaker Fdn, Cyberonics, NIH, NIBIB, NIDOCD, NINDS ISBN: 0-7803-8439-3.

This article can also be found online at http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=1403386&isnumber=30462.

Rights Information

© 2004 Institute of Electrical and Electronics Engineers (IEEE)

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Recommended Citation

Raub, C.B., E.J. Orwin, and R.C. Haskell. "Immunogold labeling to enhance contrast in optical coherence microscopy of tissue engineered corneal constructs." Proceedings of the 26th Annual International Conference of the IEEE, Engineering In Medicine and Biology Society, Vol. 1 (2004): 1210-1213.