Document Type
Conference Proceeding
Department
Physics (HMC)
Publication Date
1-1-2007
Abstract
We present 3-dimensional volume-rendered in vivo images of developing embryos of the African clawed frog Xenopus laevis taken with our new en-face-scanning, focus-tracking OCM system at 1300 nm wavelength. Compared to our older instrument which operates at 850 nm, we measure a decrease in the attenuation coefficient by 33%, leading to a substantial improvement in depth penetration. Both instruments have motion-sensitivity capability. By evaluating the fast Fourier transform of the fringe signal, we can produce simultaneously images displaying the fringe amplitude of the backscattered light and images showing the random Brownian motion of the scatterers. We present time-lapse movies of frog gastrulation, an early event during vertebrate embryonic development in which cell movements result in the formation of three distinct layers that later give rise to the major organ systems. We show that the motion-sensitive images reveal features of the different tissue types that are not discernible in the fringe amplitude images. In particular, we observe strong diffusive motion in the vegetal (bottom) part of the frog embryo which we attribute to the Brownian motion of the yolk platelets in the endoderm.
Rights Information
© 2007 International Society of Optical Engineering (SPIE)
Terms of Use & License Information
DOI
10.1117/12.701420
Recommended Citation
Hoeling, B.M., S.S. Feldman, D.T. Strenge, A. Bernard, E.R. Hogan, D.C. Petersen, S.E. Fraser, Y. Kee, J.M. Tyszka, and R.C. Haskell. Barbara M. Hoeling, Stephanie S. Feldman, Daniel T. Strenge, Aaron Bernard, Emily R. Hogan, Daniel C. Petersen, Scott E. Fraser, Yun Kee, J. Michael Tyszka and Richard C. Haskell, "Motion-sensitive 3-D optical coherence microscope operating at 1300 nm for the visualization of early frog development", Proc. SPIE 6429, 64292T (2007); http://dx.doi.org/10.1117/12.701420
Comments
Previously linked to as: http://ccdl.libraries.claremont.edu/u?/irw,374.
Art. No. 64292T
Paper presented at the following conference: Coherence Domain Optical Methods and Optical Coherence Tomography in Biomedicine XI [January 22-24, 2007, San Jose, CA] ISBN: 978-0-8194-6542-9.
Pdf acquired through Interlibrary Loan.