Graduation Year
2014
Document Type
Open Access Senior Thesis
Degree Name
Bachelor of Science
Department
Biology
Second Department
Biology-Chemistry
Reader 1
Alan Jones
Reader 2
John H Morrison
Terms of Use & License Information
This work is licensed under a Creative Commons Attribution 4.0 License.
Rights Information
© 2014 Sebastian Aguiar
Abstract
The excitatory, ionotropic glutamatergic AMPA receptor is the most common membrane-bound receptor in the central nervous system. AMPARs and the NMDA receptors are central to synaptic plasticity, memory, and mechanisms of neurodegeneration. The AMPAR is an obligate heterotetramer, composed of subunits GluA1-4. Subunit permutation determines ion conductance, trafficking and other functional characteristics. Few available antibodies are subunit-specific, disabling researchers from accurately visualizing differential AMPAR subunit distribution in the nervous system. This study sought to visualize a novel monoclonal GluA1/2/3 antibody with functional avidity for three of four receptor subunits and to characterize the ultrastructural localization of these receptors using confocal and electron microscopy.
Recommended Citation
Aguiar, Sebastian, "Characterizing a Novel Monoclonal AMPA Receptor 1/2/3 Antibody in the Hippocampus and Prefrontal Cortex of Rat, Monkey, and Human" (2014). Pitzer Senior Theses. 82.
https://scholarship.claremont.edu/pitzer_theses/82
